matrix-assisted laser . DEVELOPMENT AND APPLICATION OF AN ELECTROSPRAY IONIZATION ION MOBILITY-MASS SPECTROMETER USING AN RF ION FUNNEL AND PERIODIC-FOCUSING ION GUIDE A Dissertation by JUNHO JEON Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Electrospray ionization (ESI) - ESI is the ionization technique that has become the most popular ionization technique. Change of the electrospray ion signal with analyte concentration. Author information: (1)Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan. AND APPLICATIONS OF FIELD-INDUCED DROPLET IONIZATION MASS SPECTROMETRY AND ELECTROSPRAY MASS SPECTROMETRY Thesis by Ronald L. Grimm In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy CALIFORNIA INSTITUTE OF TECHNOLOGY Pasadena, California 2006 (Defended August 25, 2005) The traditional method of electrospray ionization involves applying an electrical charge to a substance, which creates an aerosol of charged droplets of the . ionization techniques such as electrospray ionization (ESI) [2] and matrix-assisted laser desorption ionization (MALDI) [3] have accelerated the application of MS in medicine [4]. The use of electrospray ionization mass spectrometry (ESI/MS) for the detection of neutral organic molecules becomes possible by their derivation with specific ESI/MS tagging reagents that have either proton or metal ion binding sites. We used the neutral crown ether group in several reagents to attach a metal binding site to substrate molecules. 14. 1 Reactants Products Catalytzed Most other molecular types, such as paraffins, naphthenes, aromatics, and thiophenoaromatics, are largely not ionized by ESI. We have discovered that crown ether tagging reagents become charged as their Na + or K + adducts during ESI-MS and provide a neutral and stable reagent . In electrospray ionization (ESI), an electric potential is applied to a solution inside a capillary, producing a strong electric field at the spray capillary tip. In the positive-ion mode, basic nitrogen molecules (typically pyridinic molecules) form predominantly protonated molecule ions. Electrospray ionization mass spectrometry. The atmospheric pressure ionization (API), i.e., electrospray ionization (ESI) , and atmospheric pressure chemical ionization (APCI) , together with various types of ion collision dissociation techniques , , , have significantly extended the applications of MS in biological, environmental, and pharmaceutical analysis. Electrospray ionization tandem mass spectrometric analysis of fragmentation patterns of dithiocarbamate derivatives. Electrospray ionization ( ESI) is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. This dissertation discusses two areas of study prior to detection by MS: sample preparation and ionization. Electrospray ionization mass spectrometry: a major tool to investigate reaction mechanisms in both solution and the gas phase. Interfaced with a mass spectrometer it allows the investigation of the molecular composition of liquid samples. Electrospray ionization is the ion source of choice to couple liquid chromatography with mass spectrometry (LC-MS). Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. cl997. Ideally the liquid reaching the emitter tip forms a Taylor cone, which emits a liquid jet through its apex. Eberlin MN. The application of electrospray ionization coupled to ultrahigh resolution mass spectrometry for the molecular characterization of natural organic matter. Proceedings of the National Academy of Sciences of the United States of America , 111 (42), 15184-15189. Description Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas. Journal of The American Society for Mass Spectrometry, 2012. Due to relatively fragile biomacromolecules, their structures are easily destroyed during the process of dissociation and ionization. Read "The application of electrospray ionization coupled to ultrahigh resolution mass spectrometry for the molecular characterization of natural organic matter, Journal of Mass Spectrometry (Incorp Biological Mass Spectrometry)" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. With electrospray a large variety of chemical substances can be ionized. A, The standard behavior of the electrospray ion signal with increasing analyte concentration. The book is divided into four main sections which can be characterized globally as fundamental and mechanistic aspects of the electrospray process, coupling electrospray to various mass analyzers, interfacing electrospray to separations techniques, and applications of . This work explores three significant causes of this observed . Nanospray DESI (nDESI) is a new variant of DESI, and its application to whole-cell mass spectrometry is limited. Presents major fundamental issues in such areas as immunology, biochemistry, and natural toxins. The electrospray ionization was first reported in an academic article by Masamichi Yamashita and John Fenn in 1984. KW - Electrospray ionization. 10 Citations (Scopus) The chromatograms shown in Figure 1 are typical in that the application of UniSpray results in higher signal intensities compared to Electrospray. application of electrospray ionization mass spectrometry to homogeneous catalysis Krista L. Vikse, Zohrab Ahmadi, J. Scott McIndoe∗ Department of Chemistry, University of Victoria, P.O. Fakultät für Chemie und Biowissenschaften That leads to the expulsion of an aerosol containing charged droplets that carry the molecules to be analyzed. This is a fruitful means to study compounds that are challenging to ionize molecularly, such as 3-chloropropane-1,2-diol (3-MCPD). Epub 2016 Feb 1. Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis Proc Natl Acad Sci U S A . Here, we present a proof-of-concept study to determine the feasibility of using desorption electrospray ionization mass . 4. @article{Sleighter2007TheAO, title={The application of electrospray ionization coupled to ultrahigh resolution mass spectrometry for the molecular characterization of natural organic matter. Box 3065, Victoria, BC V8W 3V6, Canada Contents 1. Electrospray ionization is today the most widely used ionization technique in chemical and biochemical analysis. The designs of several TC-ESI sources are discussed, as well as selected applications. The electrospray is created by putting a high voltage on a flow of liquid at atmospheric pressure, sometimes this is assisted by a concurrent flow of gas. Ambient ionization techniques do support whole bacterial cell analysis, which include desorption electrospray ionization (DESI). ISBN 978--471-74107-7 (cloth) 1. Electrospray ionization is a well-established technique that has existed for decades and has applications in multiple fields, including mass spectrometry and material analysis/creation. Application of Electrospray Ionization Mass Spectrometry to the Analysis of Oligodeoxynucleotides, Charles R. Iden et al. kminakat@hama-med.ac.jp In the present review we have described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules. The principles, development, and recent application of electrospray ionization-mass spectrometry (ESI-MS) to biological compounds are reviewed. High voltage is applied to a liquid supplied through an emitter (usually a glass or metallic capillary). The method introduced here, involving direct application of highresolution electrospray ionization mass spectrometry for monitoring relevant intact therapeutic protein variants, without proteolytic fragmentation and liquid chromatography separation prior to MS analysis, represents a more robust and therefore QC compatible approach for release . Research output: Contribution to journal › Article › peer-review. 16. Presents new ways of creating ions of polar and labile biopolymeric species with remarkable ease and efficiency. The name electrospray is used for an apparatus that employs electricity to disperse a liquid or for the fine aerosol resulting from this process. of: Electrospray ionization mass spectrometry. Ionic species in solution can thus be analysed by ESI-MS with increased sensitivity. Presents a wide view of the current knowledge concerning the basic aspects of electrospray ionization mass spectrometry. 2014 Oct 21;111(42):15184-9. doi: 10.1073/pnas.1408129111. Among the compounds detected and characterized in the extracts, four anthocyanin-3-(6-O- p -coumaroyl)5-O-diglucosides were identified in Clinton grape for the first time and their electrospray ionization mass spectra could be obtained for the first time together with those of cyanidin, peonidin, and malvidin 3,5-O-diglucosides. The analysis can be performed online, by feeding the liquid eluting from the LC column directly to an electrospray, or offline, by collecting fractions to be later analyzed in a classical nanoelectrospray-mass spectrometry setup. Please do not reuse or reproduce without the author's permission. Mass spectrometry is emerging as a powerful analytical tool at various key stages in the drug discovery process. Introduces better characterization of proteins and other biological molecules. 15. Author Alina D Zamfir 1 2 Affiliations 1 Aurel . Application of Electrospray Ionization Mass Spectrometry Towards the Elucidation of Free Radical Polymerization Mechanisms Zur Erlangung des akademischen Grades eines DOKTORS DER NATURWISSENSCHAFTEN (Dr. rer. Application of electrospray ionization MS/MS and matrix-assisted laser desorption/ionization-time of flight mass spectrometry to structural analysis of the glycosyl-phosphatidylinositol-anchored protein Taguchi, R.; Hamakawa, N.; Maekawa, N.; Ikezawa, H. Journal of Biochemistry 126(2): 421-429 1999 ISSN/ISBN: 0021-924X PMID: 10423539 Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. We are reporting a study of the fundamental and application aspects of positive-ion electrospray ionization (ESI+) for petroleum characterization. This study is the first demonstration, to our knowledge, of the application of desorption electrospray ionization mass spectrometry imaging (DESI-MSI) for discrimination of breast cancer and delineation of tumor margins. is the application of DESI to rapid chemical fingerprinting of Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions. Presents new ways of creating ions of polar and labile biopolymeric species with remarkable ease and efficiency. Application of Probe Electrospray Ionization Mass Spectrometry (PESI-MS) to Clinical Diagnosis: Solvent Effect on Lipid Analysis. Applications of High-Resolution Electrospray Ionization Mass Spectrometry to Measurements of Average Oxygen to Carbon Ratios in Secondary Organic Aerosols Adam P. Bateman,† Julia Laskin,‡ Alexander Laskin,§ and Sergey A. Nizkorodov*,† †Department of Chemistry, University of California, Irvine, California 92617, United States As mentioned already, electrospray existed long before its application to mass spectrometry. Hofstadler and Sannes-Lowery review the application of electrospray ionization mass . Minakata K(1), Suzuki M, Suzuki O. Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological . Eur J Mass Spectrom (Chichester), 13(1):19-28, 01 Jan 2007 Cited by: 52 articles | PMID: 17878534 Applications of Desorption Electrospray Ionization (DESI) Richard H. Perry Department of Chemistry Stanford University Mass Spectrometry September 26, 2011 Presented at the 2011 Stanford Mass Spectrometry Users' Meeting For personal use only. the development of atmospheric pressure ionization (api) techniques, especially electrospray ionization (esi), has enabled ms to detect transient species directly from the reaction vessel, which. While ESI-MS is frequently used qualitatively with some success, quantitative use of the technique has been held back by observed deviations between the gas phase measurements and known solution speciation. This Paper. Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis. Applications of capillary electrophoresis electrospray ionization mass spectrometry in glycosaminoglycan analysis Electrophoresis. Rev. Quadrupole Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. The Electrospray Ionization (ESI) is a soft ionization technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supramolecules. The application of electrospray ionization coupled to ultrahigh resolution mass spectrometry for the molecular characterization of natural organic matter Rachel L. Sleighter and Patrick G. Hatcher∗ Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA 23529, USA Received 2 February 2007; Accepted 27 March 2007 Using DESI-MSI, it is possible to discriminate between cancerous and adjacent normal tissue on the basis of the detection and specific spatial distributions of different lipid .
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